FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bw-20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bw-20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10874938
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG133370.12263977964747937No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG127920.11762825682316534No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT117760.10828567482407715No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG110320.10144425650978424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA28700.033.910211
ATGCGTG38950.027.2816546
ACATGCG39550.027.1295994
CTGACAT41500.026.9438821
CATGCGT43600.024.609425
TTACGGG10450.024.4297458
CGGGACT24800.023.100411
TGCGTGC47200.022.5119067
CGGAATA30650.021.61524468
GTGCGAG49500.021.11742610
ACATCCG42350.020.9358855
GTCCCTA34850.020.689368
TGCGAGT50800.020.57701911
CCTTACC12750.020.5715351
GAGTCGA52700.020.42431414
CGCTATG52950.020.39313129
GTCGCTA52200.020.3557827
CTATAAG44950.019.72463246
TAGTCAT48400.019.6739336
CGTGAAC16150.019.65534864
Produced by FastQC (version 0.11.2)