FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bw-20_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bw-20_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13213536
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG168000.12714234857346285No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG152510.11541952131511202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA32250.036.3844381
ACATGCG48950.029.6727474
ATGCGTG49700.029.5719476
CTGACAT53350.028.0751271
CATGCGT51950.027.8263855
TTCGATC24500.025.4891662
TGCGTGC60500.024.2915177
TGCGAGT62850.022.77945111
ACATCCG46750.022.3602355
GTGCGAG64450.022.26746710
CGGGACT29800.022.1199191
CGTGAAC20100.021.45787464
CTATAAG49750.021.21934946
GAGTCGA68950.020.7141214
TATAAGC51400.020.53818347
GTCGCTA58950.019.95596127
GTCCCTA37550.019.9365628
TAGTCAT58900.019.7397356
AGTCGAC72900.019.5917515
CGCTATG60500.019.4446929
Produced by FastQC (version 0.11.2)