FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bw-17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bw-17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9487275
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG116010.12227957975287952No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT101850.10735432460848875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG32850.027.6208254
GCCAGTA21750.027.6094251
ATGCGTG34350.027.0172946
CTGACAT37850.025.7128721
CGGGACT17800.025.0113471
CATGCGT36750.024.9712665
TGCGTGC43000.021.8221937
ACATCCG29600.020.97926155
GTCGCTA42400.020.58519227
CGCTATG43600.020.09775229
GTGCGAG45050.020.06350310
CGGAATA31850.020.0446168
GAGTCGA46000.019.72414214
TGCGAGT45800.019.65962811
TTACGGG9850.019.6133638
CTATAAG30700.019.55346
TTCGATC20450.019.2327582
CCTTACC10100.019.1352461
GCTATGA46100.018.93301830
CTACCCG22450.018.901886
Produced by FastQC (version 0.11.2)