FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bw-17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bw-17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16257904
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG192610.11847160617998481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA38350.034.827061
ATGCGTG55950.028.1176936
ACATGCG58500.027.0689724
CTGACAT62500.026.2844071
CATGCGT65450.024.1945725
TTCGATC31850.023.6140252
CGGGACT34750.023.4384271
TGCGTGC73800.021.5498777
CGTGAAC24300.021.44096864
CGGAATA49900.020.74626268
GAGTCGA78350.020.34257114
GTAAGCG16550.020.01441663
GTGCGAG78200.019.94031110
TGCGAGT78650.019.87014611
ACATCCG45850.019.86458855
AGTCACG13850.019.67802828
TAAGCGC17250.019.4024464
TAGTCAT67350.019.3628446
ACGTCTG14300.019.05873115
TAGGTAC46900.018.97791313
Produced by FastQC (version 0.11.2)