FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bs-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bs-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14371597
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG34200.023.6058794
ATGCGTG35050.022.9348986
CATGCGT36300.021.9551185
CTCGTAT12900.020.85984842
TCGTATG14750.020.11465843
TATGCCG15200.019.51916146
TGCGTGC41850.019.2895537
TGCGAGT42300.018.83967411
GCCAGTA40100.017.98841
CTGACAT50900.017.697561
GTGCGAG47300.017.13990810
CGGAATA32550.016.74925468
CGCTATG45800.016.72215529
CTATAAG41000.016.07147646
GATAGGA58200.016.06412537
TAGTCAT44000.015.9958166
GTCGCTA48350.015.76881427
ACTGACC18150.015.58620732
TATAGGG36700.015.41659960
CTATGCG48550.015.13586454
Produced by FastQC (version 0.11.2)