FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bs-2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bs-2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11280960
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14550.027.7416543
TATGCCG15950.025.7391246
ACTTAGG16700.024.58284832
CTCGTAT16850.024.15969742
AGTCACT21450.019.13909328
GTATGCC22700.018.8453145
GTCACTT22050.018.46184529
ACATGCG24400.018.3810634
CGTATGC22650.018.27773944
ATGCGTG26000.017.3826126
CGCTATG33250.017.32712629
GTAAGCG15300.017.13922763
ACGTCTG26400.016.85720615
ACCTAGG23500.016.73542610
CATGCGT26750.016.5083355
GTCGCTA33900.016.4861427
CCTATTA23900.016.16664511
ACACGTC28900.015.75708313
TAGGTAC24150.015.71358713
TACCTAG25550.015.6627089
Produced by FastQC (version 0.11.2)