FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bs-20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bs-20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11353000
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG126110.11108077160221967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA33450.030.3325371
CATGCGT34400.027.7808155
ATGCGTG35800.027.272636
ACATGCG36450.026.5969854
TGCGTGC41100.024.0903267
CTGACAT41100.024.0149561
TGCGAGT40950.023.16761811
GTGCGAG42750.022.51493510
TAGTCAT46600.021.1740426
CGCTATG45200.020.22609129
GTCGCTA46400.020.14910727
GTCCCTA32700.019.9396088
CCTATTA33150.019.87707311
TAGGTAC30550.019.53608313
CGGAATA32600.019.15865168
GTAAGCG16650.019.06502563
ACCTAGG31300.018.84752710
TAAGCGC17250.018.80226764
GTGGATA32550.018.5485824
ATACCTA35200.018.3274298
Produced by FastQC (version 0.11.2)