FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bs-20_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bs-20_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24646938
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG250360.10157854091246547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA76000.028.201551
CATGCGT75600.025.8296575
ACATGCG77850.025.4377174
ATGCGTG78500.025.314836
CTGACAT94100.022.81371
TGCGTGC90450.021.9309647
TGCGAGT89250.021.60741811
CTCGTAT23500.021.5842
TAGTCAT98450.021.2713576
CGGGACT58900.020.743541
GTGCGAG94350.020.54910310
CGCTATG98500.020.41890729
GTCGCTA100550.019.9683427
TCGTATG25700.019.8669243
GTCCCTA73550.019.7468518
CCTATTA76750.019.05841611
TATGCCG27100.018.96792646
CTACCCG67550.017.7225536
GCTATGA114550.017.61817630
ACCTAGG60750.017.49052610
Produced by FastQC (version 0.11.2)