FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bs-17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bs-17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13618558
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG42350.023.5440794
GCCAGTA31550.023.4101831
CATGCGT44300.022.5855965
ATGCGTG44200.022.5585546
CTGACAT50900.021.765951
TGCGTGC49850.020.2075427
GTCGCTA62300.020.15629827
CGGGACT28100.019.8975281
TGCGAGT50450.019.76214611
CGCTATG63400.019.75224129
GTGCGAG52850.018.79937210
CTATAAG40800.018.09508346
GCTATGA69350.018.00781830
CGCGAAT32000.017.6882271
ACATCCG41000.017.67043355
TATAGGG47100.017.65263460
CGGAATA44950.017.58009568
GTCCCTA36250.017.3204528
TAGGTAC24700.017.31897413
TATAAGC42950.017.10895347
Produced by FastQC (version 0.11.2)