FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bs-17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bs-17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14278982
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG44050.025.6886924
GTCACGT15900.025.16872629
CATGCGT45350.025.104455
ATGCGTG45850.024.755446
AGTCACG16650.024.03528
CTGACAT51800.023.3850861
GCCAGTA33800.023.1777231
TCGTATG17800.023.06369445
CTCGTAT15950.022.92723544
TATGCCG18800.021.6535548
TGCGTGC52500.021.6189177
CGGGACT29150.020.7186341
TGCGAGT53800.020.7118611
GTGCGAG53650.020.64109210
CGCTATG59700.019.93632729
GTCGCTA61200.019.44769127
CCTATTA31050.019.44353911
CACGTGA20600.019.42641631
TGAAACG21500.019.25525535
TCACGTG21550.018.73018330
Produced by FastQC (version 0.11.2)