FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bw-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bw-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19624685
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA50450.022.8479671
ACATGCG49200.022.1586134
CATGCGT52250.020.6670065
ATGCGTG55500.019.7675886
CGCTATG68550.019.02331229
GTCGCTA70150.018.73700727
TGCGAGT56450.018.63964811
ACCTAGG52100.018.01070610
TGCGTGC61150.017.8274887
CTGACAT71050.017.8264241
TACCTAG55200.017.7492039
TAGTCAT65400.017.0390056
TATGCCG19200.016.71048546
GTGCGAG63350.016.66381310
CTCGTAT19050.016.66100942
TAGGTAC57550.016.30517213
GCTATGA82300.016.13856530
GTCCCTA49450.015.7667348
CGGGACT38150.015.7404441
GTGGATA58400.015.7140964
Produced by FastQC (version 0.11.2)