FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bw-2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bw-2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9916126
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG28150.026.9628454
GCCAGTA25550.026.6108571
CATGCGT29000.026.2915235
ATGCGTG29400.026.0510336
CTGACAT32800.025.043021
TTCGATC20800.022.5586132
TGCGTGC34450.022.4313897
CTCGTAT7250.021.88900242
TGCGAGT36050.021.4359311
TCGTATG9350.021.03145843
GTGCGAG37450.020.91083710
GTGGATA30950.020.2876724
CTATAAG34800.019.82689546
CGCTATG40500.019.59191129
ACATCCG34200.019.46920855
ACCTAGG31900.019.35796710
TATAAGC35250.019.18231247
GGTGGAT32550.019.0789263
TATGCCG9050.019.0601146
CGGGACT23050.019.0158771
Produced by FastQC (version 0.11.2)