FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bw-20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bw-20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7286890
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG23300.025.0236634
ATGCGTG24750.023.1394486
CATGCGT25250.023.0911435
GTCGCTA29000.022.84033627
CGCTATG29500.022.8040429
CTCGTAT6700.022.65583642
CTGACAT28500.021.555851
TGCGAGT27250.020.63575211
GCTATGA33250.020.54356230
GCCAGTA16950.020.3619941
ACGTCTG8900.020.15638215
TGCGTGC29000.020.1042547
GTGCGAG28550.019.81695710
GGTAACT35350.018.64525669
GAGTCGA31250.018.54635214
ATCGAAG40400.018.2747946
GCAACGT37350.018.1960822
GTGGTAA37600.017.89650367
AGTCGAC32250.017.864315
TATCTCG8800.017.64148139
Produced by FastQC (version 0.11.2)