FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bw-20_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bw-20_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8342047
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG100690.12070178938095169No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG89140.10685626681316948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA23200.033.9181061
CTGACAT26400.028.238041
ACATGCG25900.028.1061134
ATGCGTG26900.027.3177816
CATGCGT28050.026.1978025
TGCGTGC31500.023.4369167
ACCTAGG25250.022.54342510
GTGGATA26000.022.1595674
TAGGTAC25350.022.04636213
TGCGAGT32550.022.04510911
TACCTAG26400.021.8227679
CGGGACT19800.021.7886121
GGTGGAT26750.021.6675013
GTGCGAG33700.021.49743710
GGATACC27450.021.4917556
TTCGATC16200.021.296632
CTCGTAT7400.020.9791842
TAGTCAT36150.020.9003686
TAAGTCG22650.020.7141919
TCGTATG8300.020.36693643
Produced by FastQC (version 0.11.2)