FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bw-17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bw-17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11725881
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG133980.11426007137544719No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT125310.10686617065276374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA28350.033.9633981
ATGCGTG41600.026.7045656
CTGACAT44500.026.3681071
ACATGCG43800.025.5208854
CATGCGT45200.024.5013245
CGGGACT24050.023.6771221
CGTAATC6700.021.62622838
TACGTAA7250.020.93713636
TGCGTGC54400.020.7371777
TTCGATC21050.020.4879062
GTCGCTA51800.020.44622227
TGCGAGT54450.020.4014311
TAGTCAT46600.020.2115556
CGGAATA36750.020.18833268
GTGCGAG55200.020.18664710
TAGGTAC27700.020.05158613
GAGTCGA55900.019.99566314
CGCTATG52850.019.77889829
GTAAGCG11050.019.35945763
GCTATGA55100.019.34681530
Produced by FastQC (version 0.11.2)