FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bw-17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bw-17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11266507
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG127490.11315840836916002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA26450.035.7523881
CGTTTCG9900.031.3627433
ATGCGTG37350.029.0967166
GTCACGT10900.028.48555229
ACATGCG39250.027.7762364
CATGCGT40100.027.2733765
CTGACAT41250.027.1082361
AGTCACG12150.025.55494128
CGGGACT23050.024.7054751
TTTCGGA12850.024.16273335
TGCGTGC47300.023.1205967
CTCGTAT11950.023.09567644
TTCGGAA12950.022.91054336
GTAAGCG10450.022.45151163
TGCGAGT48450.022.2870911
TATGCCG13700.021.9082348
GTGCGAG50250.021.35134310
GGTGGAT34150.020.9126683
ACGTTTC15000.020.69940832
TAGGTAC33700.020.67875113
Produced by FastQC (version 0.11.2)