FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bs-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bs-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6432298
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6700.034.49883342
TCGTATG7900.029.69519643
TATGCCG8550.029.05187246
ACATGCG15650.026.4535984
CATGCGT17400.023.7930375
CACCGAT11200.022.79333931
ATGCGTG18850.022.328856
CAGTCAC11550.022.10297427
CTGACAT20350.022.046791
ACGTCTG11600.022.00770415
GCCAGTA14450.021.7340161
TATAGGG17100.021.38623860
CGTATGC11300.021.37095644
TGCGAGT18900.021.1737211
TGCGTGC20300.020.9027447
ATGCCGT11750.020.55265647
GTATGCC12650.020.45353545
ACACGTC12850.019.86687713
TATCTCG12150.019.59253739
CACGTCT14050.019.39776614
Produced by FastQC (version 0.11.2)