FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bs-2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bs-2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16307127
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG32700.021.4176924
TCGTATG14300.020.26498643
ATGCGTG36500.019.6603936
CATGCGT35850.019.5358035
CGCTATG49750.018.72275529
CTCGTAT13900.018.6144242
GTCGCTA50700.018.37210527
TAGGTAC40700.017.96974413
CTTCCGA69950.017.8121241
ACCTAGG42050.017.4748210
TACCTAG44650.016.9980969
GTGGATA43000.016.9292764
GCCAGTA44150.016.8857081
TGCGTGC42450.016.8224957
GGTAACT53950.016.69429269
TAGTCAT41350.016.686926
TGCGAGT41400.016.6659511
CGGGACT30850.016.4459271
CTGACAT50250.016.140911
GTAAGCG19100.015.89684763
Produced by FastQC (version 0.11.2)