FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bs-20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bs-20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11736175
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG133830.11403204195574793No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC125360.10681503982345184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA34650.031.0741421
CATGCGT36050.025.3603425
ACATGCG36750.025.1589184
CTGACAT40400.024.772191
ATGCGTG37900.024.3955236
TGCGTGC41500.022.5280087
CGGGACT28250.022.4774551
GTCCCTA35900.021.909988
TAGTCAT48350.021.834316
GTGCGAG43150.021.58661510
TGCGAGT43050.021.55662311
GTGGATA34650.021.1080064
ACCTAGG35650.020.70884310
CCTATTA39050.020.49597411
TAGGTAC36050.020.38336813
TACCTAG38200.020.048939
CGCTATG47950.019.78543929
GGTGGAT37800.019.5317063
ATACCTA40800.018.8558588
CTATTAA42900.018.73700512
Produced by FastQC (version 0.11.2)