FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bs-20_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bs-20_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13135410
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA37950.024.8294091
ACATGCG40200.021.9709644
ATGCGTG43650.021.1038766
CATGCGT41900.021.079545
CGGGACT29700.021.0347611
CTGACAT49350.019.9330231
ACCTAGG32150.019.63649710
CGCTATG50200.019.5168729
TACCTAG34500.018.9988969
TGCGAGT47400.018.99580611
GTCCCTA36100.018.8257778
TAGTCAT49550.018.7998926
GTGCGAG46450.018.79008310
TGCGTGC47450.018.7576057
TAGGTAC32750.018.7501313
GTCGCTA52700.018.72194527
GTGGATA34650.017.9227434
ATACCTA36600.017.814548
CGCGAAT35550.017.7675271
ACATCCG46300.017.73425755
Produced by FastQC (version 0.11.2)