FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bs-17_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bs-17_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11714966
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT118370.10104169316411162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10000.027.94381944
AGTCACG11550.025.3884328
TCGTATG12750.024.35180945
GCCAGTA28450.024.2624071
ATGCGTG36700.024.0658176
CATGCGT37200.023.8350945
GTCACGT12350.023.74383529
ACGTCCG12650.023.18064132
ACATGCG38000.023.1517224
CTGACAT43400.022.107581
TGCGTGC41450.021.3897467
ACGTCTG16350.021.31093615
TTACGGG11800.021.0497788
TGCGAGT42150.020.70722211
CGGGACT26500.019.926531
GTGCGAG44150.019.84723710
TATGCCG16350.018.77910448
TCACGTC16150.018.58427630
CGCTATG56000.018.41967829
CTATAAG34150.018.1837946
Produced by FastQC (version 0.11.2)