FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_3bs-17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_3bs-17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27019679
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA80950.027.2025411
CATGCGT73800.024.2160325
ATGCGTG75750.024.002566
ACATGCG77200.023.5070464
CGCTATG109450.020.6454629
TAGTCAT104750.020.3876046
TGCGTGC89800.020.360867
CTGACAT97750.020.2322181
GTCGCTA112300.020.12154627
TGCGAGT89350.020.0387311
CTCGTAT22900.020.0363442
GTCCCTA84200.019.5435268
GTGCGAG93550.019.21279510
CTATGCG116900.018.76978554
CGGGACT56350.018.7427431
CCTATTA89450.018.58934611
ACCTAGG68900.018.37568310
TCGTATG25100.018.28016943
AGTCACG26000.017.91266628
ACATCCG105050.017.79999755
Produced by FastQC (version 0.11.2)