FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_4bw-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_4bw-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6361002
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGT16050.024.7190825
ACATGCG17150.023.7370854
ATGCGTG17700.023.3893156
GTCGCTA25150.022.63333727
CGCTATG25100.022.5409829
CGGGACT12250.021.1296221
TGCGTGC19900.020.9762637
TGCGAGT18900.020.62620411
TTACGGG8250.020.0716538
GTGCGAG20750.019.78448310
CGCGAAT17800.019.7763771
CTGACAT23650.019.7001341
GCTATGA29550.019.26323130
GCCAGTA16250.019.1141821
GGTAACT29600.018.6529369
CTATAAG19150.018.5555846
TCGTATG5050.018.44542743
GTGGTAA30800.018.26230867
CCCTGTG30600.018.1542763
TTCGTAC26400.017.64110220
Produced by FastQC (version 0.11.2)