FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_4bw-2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_4bw-2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10855796
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13200.022.73758144
TCGTATG14750.021.98521845
TATGCCG15900.020.82912648
CGTATGC18550.018.22548746
ACGTCTG21100.017.82136515
CTATAAG27850.017.21815346
CGCTATG30150.016.93453629
CATGCGT25250.016.806095
GTCGCTA31750.016.5157727
TATAAGC29300.016.24831847
ACATGCG26800.015.4478984
ATGCGTG28200.015.4149586
GTTCGTA28250.015.14259113
AGTCACA24250.014.9374528
ACATCCG33400.014.87424855
TTCGTAC29700.014.6355520
TGCGAGT28550.014.62097111
GTCCCTA21650.014.5003498
CAGTCAC25300.014.31751627
GTATGCC25400.014.12526447
Produced by FastQC (version 0.11.2)