FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_4bw-20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_4bw-20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8647580
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG92050.10644596522957868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG27450.028.1528034
ATGCGTG28200.027.893426
GCCAGTA23600.027.6408671
CATGCGT29450.026.3580475
CTGACAT36150.023.2961081
TGCGAGT33950.022.55857311
TGCGTGC34700.022.4685427
CGGGACT15450.021.6693121
GTGCGAG35800.021.10362210
CGGAATA22600.020.46116868
CGCTATG34150.020.10299529
ACATCCG33750.019.62641355
CTCGTAT8450.019.59715342
TATAAGC33950.019.408547
GAGTCGA39500.019.3889514
CTATAAG33450.019.2861946
GTCGCTA35450.019.26847627
TAGTCAT35200.018.3279936
AGTCGAC42000.018.31698415
TTACGGG8600.018.0513618
Produced by FastQC (version 0.11.2)