FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_4bw-20_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_4bw-20_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10719047
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG120250.11218348049038315No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT108030.10078321328379286No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG107320.10012084096655234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA29800.029.8796751
ATGCGTG33850.028.6391416
ACATGCG35000.028.0928154
CATGCGT36450.026.596675
CTGACAT41250.025.0161041
TGCGTGC41150.023.7254687
TGCGAGT42700.022.78355211
GTGCGAG43800.022.36878610
TAGTCAT40700.021.4456146
CGCTATG45650.020.25322729
CTATAAG38500.020.07185646
CGGGACT22800.019.8293691
GTCGCTA46900.019.78698527
TATAAGC39800.019.6762847
CTCGTAT9600.019.40549742
ACATCCG39200.019.36212555
GAGTCGA50950.019.09436214
GTAAGCG12700.019.01793963
AGTCGAC51900.018.74484815
CGGAATA33850.018.55421668
Produced by FastQC (version 0.11.2)