FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_4bs-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_4bs-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11336072
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG29100.026.5577184
ATGCGTG30900.025.345636
CATGCGT30650.025.214675
TCGTATG9650.024.66781443
GCCAGTA29000.024.040441
TGCGTGC34450.022.7316117
CTCGTAT9050.022.49118442
CTGACAT38650.022.4136831
TGCGAGT34950.021.5182411
TATGCCG11100.020.8234746
CGCTATG38650.020.61899829
GTCGCTA40500.019.67705527
GTGCGAG39400.019.26282710
TTCGATC24500.018.0256562
CGGGACT24000.017.9757821
TAGGTAC32200.017.67768913
TTACGGG12500.017.6629628
GCTATGA46550.017.56426230
ATTAGGG21350.017.4526586
GTCCCTA29950.017.392978
Produced by FastQC (version 0.11.2)