FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_4bs-2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_4bs-2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9793151
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8800.030.97047443
CTCGTAT9250.028.34492542
CATGCGT25500.026.382215
ATGCGTG26450.025.9563836
ACATGCG25850.025.7580834
TATGCCG10250.025.24286346
GCCAGTA25200.024.1027371
TGCGAGT28750.022.55872511
CTGACAT33500.022.1486821
TGCGTGC31050.021.8876537
AGTCACG12900.021.66167828
GTGCGAG31000.021.4778210
CGCTATG30500.020.47272129
TTCGATC19600.020.2425482
CTACACG7450.019.91162712
CACGGCT13900.019.60700631
CGCGAAT28250.019.3015821
CTATAAG31200.019.23959446
TAGTCAT32100.018.8083116
GTCGCTA33200.018.70395527
Produced by FastQC (version 0.11.2)