FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_4bs-20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_4bs-20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11047815
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG112070.10144087315003011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA33100.029.089071
CATGCGT32050.026.265385
ATGCGTG32350.026.2352186
ACATGCG32300.025.8484654
CTCGTAT8150.025.39801842
TCGTATG8450.025.31285743
ACCTAGG34400.023.96865510
GTGGATA35250.023.1958944
TACCTAG36400.023.1255849
TGCGTGC37750.022.6641247
TAGGTAC36700.022.46643313
CTGACAT40950.021.9958251
TAGTCAT42750.021.7090116
GGATACC38800.021.6071976
TGCGAGT39000.021.58387611
GTACCCA38850.020.86791816
GTGCGAG40400.020.66513410
TTCGATC26900.020.6491152
ATACCTA41950.020.1482968
GGTGGAT41450.019.9762743
Produced by FastQC (version 0.11.2)