FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_4bs-20_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_4bs-20_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18561437
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG195230.10518043403643802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA53850.030.3808251
ATGCGTG53700.025.1204856
ACATGCG54550.024.729194
CATGCGT53650.024.6295765
TAGTCAT72600.022.0499156
CTGACAT66750.021.35531
TGCGTGC65050.020.948067
CGCTATG65400.020.09778429
GTCGCTA66250.019.89227
TGCGAGT66150.019.81766111
GTGCGAG68850.019.04029310
CTATAAG66050.018.75092146
CGGGACT39050.018.7379361
CGGAATA48350.018.69979768
GTCCCTA53250.018.463728
CGCGAAT60350.018.4155851
TATAAGC67650.018.10331247
ACATCCG68600.017.75265355
TCATATG91050.017.7320889
GCTATGA74600.017.66538830
Produced by FastQC (version 0.11.2)