FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_3bw-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_3bw-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3807233
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT3500.028.5851739
TCGTATG4350.025.37849445
CTCGTAT4150.024.10765844
TATGCCG5700.019.97330548
TTCCGTA5650.019.53945236
ACATGCG9700.019.2065774
ATGCGTG9350.019.1873056
GTCCCTA7400.019.1142488
ACGTCTG6200.018.91874515
CTATAAG10800.018.20774746
CATGCGT9850.017.8633085
ACCTAGG9150.017.72074110
TAGGTAC9550.017.33975613
TATAAGC11650.016.87950747
CGGGACT6550.016.8606191
TGCGAGT10250.016.82872611
TCCGTAT6600.016.72695537
CCTATTA8500.016.64064211
CTATGCG12300.016.54868354
TACCTAG10600.016.2730629
Produced by FastQC (version 0.11.2)