FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_3bw-2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_3bw-2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4271476
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA12050.025.4911291
CTCGTAT2500.024.83937344
CATGCGT10700.024.5043415
ACATGCG10650.024.2954464
ATGCGTG11150.023.51516
TTACGGG2900.022.6020838
TTCGATC9650.020.7357142
GTCCCTA11150.020.729698
TGCGTGC12500.020.698757
ACCTAGG13650.020.4712910
GTGGATA13700.020.3975334
TAGTCAT14600.020.0851086
CGCTATG12850.019.86650529
TACCTAG14100.019.8179519
TGCGAGT13250.019.78748311
TCGTATG3500.019.71378745
CCTATTA11900.019.71309511
CGGAATA10000.019.6704968
CTATAAG13550.019.35006546
TATAGGG12400.018.64109460
Produced by FastQC (version 0.11.2)