FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_3bw-20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_3bw-20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787152
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG54280.11338683208722013No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG52290.10922987195727231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTG15150.029.8324536
CATGCGT15650.028.658895
ACATGCG15550.027.9557084
GCCAGTA17650.026.2012481
CTGACAT18850.024.5332641
TGCGTGC19250.023.1181477
CTATAAG16800.022.99915546
ACGTCTG4400.021.95328115
ACATCCG18250.021.9284255
TATAAGC18300.021.49101347
TGCGAGT20250.020.95434811
GTGCGAG21200.020.01535610
GTCGCTA20400.019.95493927
CCCTGGT21300.019.92867367
CGCTATG20400.019.7858329
ACCTAGG12850.019.32962410
TAGTCAT23750.019.0299666
TAGGTAC13250.018.7460913
TAAGCAA21450.018.65646249
GATCCCT21350.018.4249164
Produced by FastQC (version 0.11.2)