FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_3bw-20_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_3bw-20_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9136753
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG109660.12002075573237013No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG101010.11055349750617095No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC93200.10200560308459691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA26300.034.9090961
ATGCGTG29050.027.5525656
CTGACAT31700.027.3292181
CATGCGT29600.027.2737165
ACATGCG30050.027.094914
CTCGTAT7650.024.80316742
TGCGTGC32650.024.5131347
TGCGAGT33350.023.7919911
TTCGATC17950.023.4494252
TAGTCAT38950.023.3838836
TTACGGG8450.023.2709718
TCGTATG8350.023.13702443
GTGCGAG34600.022.83262310
CTACACG7150.021.7122712
TATGCCG9600.020.84277546
GTCCCTA27900.020.4021598
CCTATTA28400.019.80012911
CGGGACT21300.019.607331
GTTAGTT21150.019.4200041
CGGAATA25850.019.35642268
Produced by FastQC (version 0.11.2)