FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_3bs-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_3bs-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9106949
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7400.025.17461842
ACATGCG21200.024.2487534
CATGCGT22650.023.4580295
ATGCGTG23250.023.4462346
TCGTATG8450.023.27121243
GCCAGTA25200.022.6007461
TGCGAGT23700.021.68884311
ACCTAGG25500.020.96959310
GTGGATA26500.020.180174
TAGGTAC26650.019.80581313
GTAAGCG10650.019.43868463
TACCTAG27550.019.15889
TGCGTGC28950.018.7089547
GTCCCTA24100.018.6090748
GGATACC29500.018.5957666
GTGCGAG28200.018.59486210
TAGTCAT29000.018.44056
CTATGCG34200.018.1576354
TATGCCG11050.018.10773746
CTTCCGA35800.017.7408391
Produced by FastQC (version 0.11.2)