FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_3bs-2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_3bs-2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10669337
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA29700.025.5636141
CTCGTAT8650.024.32894142
TCGTATG9300.023.7414143
ATGCGTG26750.023.6016796
ACATGCG25900.023.577144
CATGCGT25650.023.403435
TATGCCG10000.022.07909846
TTACGGG11900.021.1628028
CGCTATG35700.020.68011729
GTCGCTA36400.020.18773827
TGCGAGT30050.019.9758911
TGCGTGC31000.019.9199667
TAGTCAT37600.019.7272346
CTGACAT36350.019.4628071
GTCCCTA26500.019.1367748
CTATAAG37200.019.10405746
CGCGAAT35750.018.920651
GTGCGAG32600.018.62483210
GTAAGCG13250.018.4880363
TATAAGC39150.018.32875347
Produced by FastQC (version 0.11.2)