FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_3bs-20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_3bs-20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10355670
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG124820.12053300269321057No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC109630.10586470986425793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8750.032.33061242
TCGTATG10200.030.10203643
GCCAGTA31600.029.4904881
TATGCCG11950.025.98235746
CATGCGT30200.024.9043055
ACATGCG31800.023.9767364
ATGCGTG31150.023.9232736
ACGTCTG15100.022.61819515
CTGACAT37300.022.1153771
AGTCACT14700.021.35639828
TAGTCAT43650.021.0243366
CGGGACT21300.020.9033261
GTGGATA31250.020.5346744
TGCGTGC36150.020.517567
ACCTAGG30850.020.46404510
TGCGAGT37100.020.3643211
CGTATGC15350.020.22740644
GTCGCTA39550.019.97528327
CGCTATG39600.019.95006229
TACCTAG32550.019.9251869
Produced by FastQC (version 0.11.2)