FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir108_3bs-20_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir108_3bs-20_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13275204
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG144110.10855577059305453No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC133310.10042030239233989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA41800.028.237881
CTCGTAT12100.027.0857342
ATGCGTG34750.025.1182446
TCGTATG13250.024.9952643
CATGCGT35250.024.7618645
AGTCACG13800.024.2488828
ACATGCG37600.023.7647844
TGCGTGC41000.021.6246917
CGTATGC15350.021.1262244
CGCTATG50900.021.07859629
TAGTCAT55750.020.8549236
TGCGAGT42350.020.60951611
GTCCCTA41950.020.3948448
TATGCCG16700.020.24480446
GTGCGAG43700.020.13072610
CTGACAT53200.019.7216951
GTCGCTA54750.019.65936727
CGGGACT30900.019.657871
GTCACGA18200.018.76561529
ACGTCTG18800.018.7172215
Produced by FastQC (version 0.11.2)