FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25084539
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA14650.044.50953759
CGTATCA16400.041.2240862
TCGCCGT18050.036.3166158
CCGTATC18650.035.1464261
CGTCTAG33200.029.687181
GTGGTCG22350.029.6427554
AGCGATA22150.026.77848469
CGACGAC21850.026.3570269
TTGACGG37900.024.58747331
TCGGTGG27650.024.46015251
CTACGGT25250.023.91147813
GATCTCG28550.023.80756847
ATCTCGG29050.023.279448
CGGTGGT29550.023.12096652
TCTCGGT30750.022.5536249
AGCGATC518900.022.34319569
TACGGTG26750.021.92625414
TGACGGC42200.021.83623332
GTCGCCG31400.021.42816557
TCCGCAA28450.020.74301733
Produced by FastQC (version 0.11.2)