FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31926030
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA32050.051.994259
CGTATCA32000.051.95833662
CCGTATC34650.047.8909961
TCGCCGT35450.046.71518758
GTGGTCG40700.042.05072454
GTCGCCG46200.036.96924657
CGGTGGT48000.035.65556752
TCGGTGG48850.035.035151
GGTCGCC50000.034.0912756
TCTCGGT49900.034.01835649
GATCTCG50400.033.47518547
CGTCTAG38750.033.085521
ATCTCGG51800.032.3043348
GCCGTAT55300.030.19579760
TGGTCGC57750.029.8749855
CTCGGTG58450.029.27935850
AGCGATA22650.026.49203169
TTGACGG47900.024.78616331
CGACGAC17900.023.11864769
CTACGGT30050.022.38819913
Produced by FastQC (version 0.11.2)