FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27374545
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1184280.4326208892239122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA31950.053.7770459
CGTATCA32400.053.55335662
TCGCCGT33600.050.520158
CCGTATC35100.048.85027361
GTGGTCG39100.044.2127854
GTCGCCG41950.041.12544357
TCGGTGG45450.038.9466751
TCTCGGT46600.038.0571549
GATCTCG46400.037.9223647
ATCTCGG46250.037.6725648
GGTCGCC45800.037.5940256
CGGTGGT47850.036.8487452
CTCGGTG51400.034.77301850
TGGTCGC54750.032.20514355
GCCGTAT54900.030.98145960
CGTCTAG36100.029.5938991
GTAGATC73550.024.57871644
CTACGGT25700.024.16445413
AGCGATA19600.024.10457869
TACGGTG27000.023.12918714
Produced by FastQC (version 0.11.2)