FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33032219
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA38400.053.8201559
CGTATCA39950.051.8066762
TCGCCGT40650.050.92605258
CCGTATC41850.049.37947561
GTGGTCG48900.043.39752654
GTCGCCG52600.040.4095357
GATCTCG54800.037.83988647
TCGGTGG58300.036.69637351
TCTCGGT58750.036.47032549
CGGTGGT58500.036.15807352
ATCTCGG57350.036.1574948
GGTCGCC60050.035.28180356
TGGTCGC62250.034.2567855
GCCGTAT61250.033.6282160
CTCGGTG68700.031.34037450
CGTCTAG39150.026.1435971
CTACGGT31600.024.01953113
TACGGTG33250.022.62064214
GTAGATC94900.022.3942344
AGCGATA22450.022.27271869
Produced by FastQC (version 0.11.2)