FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29332468
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG38500.029.9861831
CGTATCA13200.025.34775462
CTACGGT29550.025.21868713
CGCCGTA13200.025.09217559
TTGACGG42950.024.027731
CCGTATC14000.023.65716661
TACGGTG32900.022.54661614
TCCGCAA33950.021.34780933
CCGACGC84750.021.19830169
AGCGATC560950.021.18332969
TGACGGC49350.021.12095632
CCTACGG35350.020.88402612
TCGCCGT17200.019.85852658
ACGGTGA38450.019.65273315
GACGGCT50000.019.39590633
GACGCAC40700.019.2302826
ACGCACC39050.019.1615187
CTAGACG41200.019.072133
GTCCGCA38700.018.90682832
GGTACGA35400.018.23010418
Produced by FastQC (version 0.11.2)