FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28922468
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA23150.049.0339759
CGTATCA23450.048.68963662
TCGCCGT24650.046.3300258
CCGTATC26400.042.8635661
GTGGTCG30050.038.46742254
GTCGCCG32200.036.1127757
TCTCGGT34700.034.0055949
GATCTCG34000.033.69056347
ATCTCGG34450.033.45096248
CGGTGGT35950.032.72988552
TCGGTGG36700.032.1553251
CGTCTAG41500.031.8051221
AGCGATA17700.030.3935769
CTCGGTG41000.029.11821250
TTGACGG47200.025.95784631
TGGTCGC48500.024.75896855
GCCGTAT46300.024.44161860
TGACGGC52500.023.46828832
CGACGAC16500.023.4080169
GGTCGCC52150.022.43061456
Produced by FastQC (version 0.11.2)