FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28268940
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA21200.050.12439359
CGTATCA23150.046.93617262
TCGCCGT23300.045.60666358
CCGTATC24750.044.32607361
GTGGTCG28000.038.20236254
GTCGCCG31600.034.28630457
TCGGTGG34150.032.4340951
TCTCGGT35150.031.80309149
CGGTGGT34700.031.32336452
GATCTCG35200.031.26821347
CGTCTAG33350.029.96931
ATCTCGG37300.029.87791348
GGTCGCC37150.029.07196856
TGGTCGC41100.026.86572855
CTCGGTG41950.026.73131450
GCCGTAT43900.024.51982960
CGACGAC16750.024.50049869
CTACGGT26200.024.36222813
TTGACGG37650.024.29159231
TACGGTG27500.023.08583514
Produced by FastQC (version 0.11.2)