FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26160718
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA20500.049.31118459
CGTATCA21200.047.6741362
TCGCCGT21650.046.05418458
CCGTATC22400.044.35596561
GTGGTCG24650.041.15438554
GTCGCCG28250.036.03104457
GATCTCG30300.033.8189147
TCTCGGT31200.033.5072549
TCGGTGG30850.033.44292451
CGGTGGT30950.033.00040452
ATCTCGG32350.031.56929448
CGTCTAG33250.031.3037091
GGTCGCC32800.031.24456
CTCGGTG39450.026.6759750
CTACGGT26300.026.10526813
TACGGTG26850.026.08520314
TTGACGG38300.025.95264831
CGACGAC12850.025.76283669
TGGTCGC41550.024.99680555
GCCGTAT41550.024.24572660
Produced by FastQC (version 0.11.2)