FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31927243
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA38550.058.08473659
CGTATCA40150.056.27282362
TCGCCGT42150.052.9599758
CCGTATC42300.052.85008261
GTGGTCG47700.047.6719854
GTCGCCG52450.042.95915257
TCTCGGT53550.042.65301549
GATCTCG53600.042.16190747
TCGGTGG55000.041.72097451
CGGTGGT55350.041.2752
ATCTCGG56200.040.57983448
GGTCGCC59200.038.06189356
CTCGGTG62950.036.12135750
TGGTCGC65550.034.90120355
GCCGTAT64300.034.60818560
CGTCTAG43300.033.588011
CGACGAC23500.031.1105869
CTACGGT30600.028.29938313
GTAGATC84400.027.18225344
TTGACGG51250.026.53325331
Produced by FastQC (version 0.11.2)