FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39249741
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA393090.10015097934022035Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA54650.055.93550559
CGTATCA55850.054.8447762
TCGCCGT57100.053.65590758
CCGTATC56800.053.6316961
GTGGTCG64750.048.9214854
GTCGCCG70550.044.16423857
GATCTCG71800.043.96873547
TCGGTGG74250.043.35914651
CGGTGGT74300.042.9119952
TCTCGGT74650.042.89219349
ATCTCGG75950.041.52101548
GGTCGCC77100.040.32380756
CTCGGTG82900.038.83330550
GCCGTAT84050.036.16332660
TGGTCGC87900.035.8802255
CGTCTAG50000.030.121141
GTAGATC109650.029.26057644
CTACGGT38450.024.5857713
CCGACGC102550.023.67345269
TTGACGG60600.023.5785431
Produced by FastQC (version 0.11.2)