FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39365059
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA20050.033.383759
CGTATCA20700.031.66198362
CGTCTAG47750.028.7954041
TCGCCGT24950.027.10381558
CCGTATC24550.026.2791861
CTACGGT39450.023.70078713
TTGACGG55700.022.80217631
GTGGTCG31200.022.78263554
TACGGTG42650.021.68048314
TCCGCAA43300.020.64289533
AGCGATC725800.020.46774369
TGACGGC64550.020.0496632
CCTACGG48250.019.23377412
GACGCAC51400.019.1849986
CTAGACG51150.019.1345083
CCGACGC106750.019.05879269
GACGGCT66350.019.03701833
CGACGAC25900.018.90600869
TCGGTGG38800.018.76469451
AGCGATA30550.018.73734969
Produced by FastQC (version 0.11.2)