FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n02_mb77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n02_mb77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36983277
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA27500.050.30954759
TCGCCGT29800.046.65808558
CGTATCA29750.046.03082762
CCGTATC32700.041.88500661
GTGGTCG39500.035.46726254
GTCGCCG42000.033.35480557
GATCTCG42600.032.80153347
ATCTCGG43500.032.20223648
CGGTGGT44650.031.06737152
TCTCGGT45400.030.93102649
TCGGTGG45800.030.43797751
CGTCTAG47300.029.4364341
AGCGATA22850.025.95760769
CTCGGTG55300.025.64439650
CGACGAC20100.025.3914869
TGGTCGC59200.024.07292255
TTGACGG55350.023.38292531
GCCGTAT59250.023.34998160
GGTCGCC60450.023.1751256
CTACGGT38050.023.12140713
Produced by FastQC (version 0.11.2)